We have
generated the draft sequence of a desi(ICC4958) type chickpea genome using next
generation
sequencing platforms, bacterial artificial chromosome end sequences and a
genetic map. The
520 Mb assembly covers 70% of the predicted 740 Mb genome length and more
than 80% of
the gene space. Genome analysis predicts the presence of 27,571 genes and
210 Mb as repeat
elements. The gene expression analysis performed using 274 million RNA-Seq
reads
identified several tissue-specific and stress-responsive genes. Although
segmental duplicated
blocks are observed, chickpea genome does not exhibit any indication of
recent whole
genome duplication. Nucleotide diversity analysis provides an assessment of
a narrow
genetic base within the chickpea cultivars. We have developed a resource
for genetic markers
by comparing the genome sequences of one wild and three cultivated chickpea
genotypes.
The draft genome sequence is expected to facilitate genetic enhancement and
breeding to
develop improved chickpea varieties.
For Genome Sequence:Click here
For downloading the paper from The Plant Journal site: Click here
Chickpea Advanced draft genome.
Further, we reported an advanced version of the ICC 4958 genome assembly (version 2.0) generated using additional sequence data and an improved genetic map. This resulted in 2.7-fold increase in the length of the pseudomolecules and substantial reduction of sequence gaps. The genome assembly covered more than 94% of the estimated gene space and predicted the presence of 30,257 protein-coding genes including 2230 and 133 genes encoding potential transcription factors (TF) and resistance gene homologs, respectively.
For Advanced draft genome (Draft 2) sequence: Click Here
For downloading the paper from Scientific Reports:
Click here